North Jersey Chromatography Group in Progress

Dr. Edward G. Franklin (Presenter)

Supercritical fluid chromatography (SFC) is an effective means to separate enantiomers, and it is often the preferred technology for such separations in pharmaceutical and other settings. Its advantages over liquid chromatographic approaches are well-documented in terms of productivities, solvent consumption, operating costs, and environmental impacts. The use of low-viscosity carbon dioxide also gives it significant speed advantages, which can be particularly helpful when developing chiral methods. Because it is difficult to predict which chiral stationary phases (CSPs) will best resolve enantiomers of interest, it is often necessary to screen compounds on many different columns with several different mobile phases. This screening step can be time consuming but is aided by the speed of SFC in combination with automated approaches that employ state-of-the-art instrumentation. Modern column technologies using small fully porous particles (FPPs) or superficially porous particles (SPPs) have also helped to accelerate the screening process and quickly identify chiral hits. In this study, practical approaches to fast chiral screening in SFC with polysaccharide-based and Pirkle-type CSPs are discussed. The influences of instrument volume and hardware constraints (e.g. maximum pumping pressures) are considered in the rationalization of column dimensions for fast chiral screening. Experimental results obtained using columns packed with 3- and sub-2-µm FPPs and 2.7-µm SPPs are examined. Advantages and disadvantages of small-particle approaches and alternative particle architectures are highlighted.

Dr. Mirlinda Biba/Brian Domanski (Presenters)

The Enabling Technologies Consortium (ETC) is a network of pharmaceutical and biotechnology companies that collaborate in precompetitive space with a goal to identify, evaluate, develop, and improve scientific tools and technologies for pharmaceutical drug discovery and development. Here we present the cross-pharma ETC collaboration project for the development of next generation preparative supercritical fluid chromatography (SFC) instrument. Participating members from ten pharmaceutical companies collaborated with Shimadzu by providing feedback and suggestions for both the hardware and software that helped with the development of semi-preparative SFC instrument. The first instrument prototypes were installed and tested at member company sites and feedback was provided to improve and refine the final product features.

Dr. Edward G. Franklin earned a B.S. in Biochemistry in 2006 and M.S. in Forensic Science in 2007 from Duquesne University in Pittsburgh, PA. In 2012, he received a PhD in Analytical Chemistry from The University of North Carolina at Chapel Hill. In over 15 years of separations-related experience, he has conducted research ranging from fundamental investigations of the kinetic performance of packed-bed HPLC columns, instrument and column development for ultra-high pressure separations of complex samples, and column stationary phase development for application-specific methods. In his current position as the Technical Director of Chromatography Applications at Regis Technologies, Inc., he develops new products and methods for chromatography solutions related to chiral and achiral separations using reversed-phase, normal phase, and supercritical fluid approaches.

Dr. Mirlinda Biba is a Principal Scientist in the Analytical Research & Development at Merck & Co., Inc in Rahway, NJ. She joined Merck in 2001 and her research work focused on developing and improving analytical and preparative chiral separations for the development and manufacture of pharmaceuticals for use in pre-clinical and clinical studies. She also led the ETC team for cross-pharma collaboration by ten major pharmaceutical companies for the development of next generation preparative SFC instrument. She received a B.A. in chemistry from Rutgers University-Newark, a M.S. in chemistry from Stevens Institute of Technology, and Ph.D. in analytical chemistry from Drexel University in Philadelphia, PA. Her research work has resulted in over 40 publications and numerous presentations as invited speaker at different conferences.

Click here for more info

North Jersey Chromatography Group

Upcoming Zoom Meeting

June 9, 2022

7:00 - 9:00 PM

Free

See Below for More Details

high-throughput chiral screening using Supercritical fluid chromatography with 3- and sub-2 mcron fully porous particles and 2.7 micron superficially porous particles

Enabling Technologies Consortium (ETC): Development of Next Generation Preparative SFC Instrument

SPEAKERS March 22

Dr. Edward G. Franklin

Technical Director of Chromatography Applications
Regis Technologies

Dr. mirlinda biba

Principal Scientist
Merck

Brian Domanski

SHIMADZU
Strategic Collaborations Scientist | Chromatography and Informatics Specialist

For upcoming events click below:

njcg.webmaster1@gmail.com

www.NJCG.ORG